Journal: Frontiers in Immunology

Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment

doi: 10.3389/fimmu.2026.1932283

Figure Lengend Snippet: Bone marrow progenitor chimerism 14 months post-allograft. Peripheral blood DNA from both the donor and the recipient, collected before the allograft, were genotyped with KMR Type (DenDx ® ) and analyzed using KMR Engine software (GenDx ® ) according to the manufacturer’s instructions. The markers KMR040 and KMR043 were specific to the recipient and the donor, respectively, and were therefore selected to genotype the BFU-E and CFU-GM colonies in order to identify the progenitor’s origin. CD34 + cells were purified from patient’s bone marrow using the CD34 MicroBead Kit (human) and plated at a concentration of 250 cells/mL in a methylcellulose-based medium with recombinant cytokines (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulocyte/monocyte differentiation. We obtained 44 BFU-E colonies and 24 CFU-GM from 1,500 CD34 + HSPCs. After 14 days, colonies were scored and collected individually (24 individual BFU-E and CFU-GM colonies, respectively). Individual colonies were genotyped by qPCR using KMRtype (GenDx). For each colony, both markers were tested alongside an internal control. Colonies that had insufficient internal control or displayed a mixed profile, with detection of both donor and recipient markers (Ct<40), were excluded from the analysis. The qPCR results were interpretable for 17 out of 24 BFU-Es and 5 out of 8 CFU-GMs tested.

Article Snippet: Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec).

Techniques: Software, Purification, Concentration Assay, Recombinant, Stem Cell, Control, Analysis

Journal: Frontiers in Pediatrics

Article Title: PROX1-associated lymphatic reprogramming signatures in pediatric adamantinomatous craniopharyngioma: a comparative study with adult cases

doi: 10.3389/fped.2026.1865684

Figure Lengend Snippet: Spatial distribution and co-expression of endothelial and lymphatic markers in pediatric ACP tissues. Representative IHC staining of serial sections from pediatric ACP tissues demonstrating the spatial distribution of CD34, PROX1, and LYVE1. All three markers display overlapping expression that clearly delineates the same ring-like structures, indicating a distinct lymphatic phenotype within the tumor microenvironment. Scale bar, 50 µm.

Article Snippet: For IHC, primary antibodies against PROX1 (Rabbit mAb, ABclonal, Wuhan, China), Nestin (Rabbit mAb, ABclonal), CD34 (Rabbit PolymAb, ABclonal), and LYVE1 (Rabbit mAb, ABclonal) were employed, followed by incubation with appropriate secondary antibodies (Zhongshan Golden Bridge Biotechnology, Beijing, China).

Techniques: Expressing, Immunohistochemistry